Product Detail
Product NameCortactin(Ab-421) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total Cortactin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.419~423 (P-I-Y-E-D) derived from Human CORTACTIN.
Target NameCortactin
ConjugateUnconjugated
Other NamesAmplaxin; CTTN; EMS1; SRC8;
Accession NoSwiss-Prot: Q14247
NCBI Protein: NP_005222.2
Uniprot
Q14247
Gene ID
2017;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 85kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin(Ab-421) Antibody #21263(left) or the same antibody preincubated with blocking peptide(right).
Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Salomon AR, et al. (2003) PNAS ; 100(2): 443-448
Huang C, et al. (1998) J Biol Chem ; 273(40): 25770-25776.
If you have published an article using product 21263, please notify us so that we can cite your literature.
Yes
