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Cortactin(Ab-466) Antibody#21264

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Product Detail

Product NameCortactin(Ab-466) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total Cortactin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.464~468 (P-V-Y-E-T) derived from Human CORTACTIN.

Target NameCortactin

ConjugateUnconjugated

Other NamesAmplaxin; CTTN; EMS1; SRC8;

Accession NoSwiss-Prot: Q14247
NCBI Protein: NP_005222.2

Uniprot Q14247

Gene ID 2017;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 85kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 3T3 and C2C12 cells using Cortactin(Ab-466) Antibody #21264.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin(Ab-466) Antibody #21264(left) or the same antibody preincubated with blocking peptide(right).

Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193
Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393

If you have published an article using product 21264, please notify us so that we can cite your literature.

et al,Involvement of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells. , (2009),
PMID: 19479281
et al,CapsaicIn attenuates cell migration via SIRT1 targetIng and Inhibition to enhance cortactIn and β-catenIn acetylation In bladder cancer cells. In Am J Cancer Res on 2019 Jun 1 by Islam A, Yang YT, et al..PMID:31285950 , (2019),
PMID: 31285950
et al,Involvement of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells. In Cell Tissue Res on 2009 Jul by Nobuo Terada, Nobuhiko Ohno, et al..PMID: 19479281 , (2009),
PMID: 19479281

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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