Product Detail
Product NameCREB(Ab-129) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total CREB protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.127~131 (I-L-S-R-R) derived from Human CREB.
Target NameCREB
ConjugateUnconjugated
Other NamesCREB-1; CREB1;
Accession NoSwiss-Prot: P16220
NCBI Protein: NP _004370.1
Uniprot
P16220
Gene ID
1385;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela and HT29 cells using CREB(Ab-129) Antibody #21265.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-129) Antibody #21265(left) or the same antibody preincubated with blocking peptide(right).
CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .
Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.
If you have published an article using product 21265, please notify us so that we can cite your literature.
et al,Hydrogen sulfide decreases β-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption
, (2015),
PMID:
Yes
