Product Detail
Product Namep62Dok(Ab-362) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p62Dok protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.360~364 (P-I-Y-D-E) derived from Human p62Dok.
Target Namep62Dok
ConjugateUnconjugated
Other NamesDOK1
Accession NoSwiss-Prot: Q99704
NCBI Protein: NP_001372.1
Uniprot
Q99704
Gene ID
1796;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 62kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells using p62Dok(Ab-362) Antibody #21268 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok(Ab-362) Antibody #21268(left) or the same antibody preincubated with blocking peptide(right).
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Zhou Songyang, et,al. (2001) J. Biol. Chem ; 276: 2459 - 2465.
Jean-Guy N
If you have published an article using product 21268, please notify us so that we can cite your literature.