Product Detail
Product Namep56Dok-2(Ab-299) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p56Dok-2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.297~301 (G-E-Y-A-V) derived from Human p56Dok-2.
Target Namep56Dok-2
ConjugateUnconjugated
Other NamesDOK2
Accession NoSwiss-Prot: O60496
NCBI Protein: NP_003965.2
Uniprot
O60496
Gene ID
9046;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from JK cells using p56Dok-2(Ab-299) Antibody #21270.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Ab-299) Antibody #21270(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Ab-299) Antibody #21270.
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation
Feng Cong, et,al. (1999) Mol. Cell. Biol; 19: 8314 - 8325.
Serge Lemay, et,al. (2000) Mol. Cell. Biol; 20: 2743 - 2754.
Ute Schaeper, et,al.(2000) J. Cell Biol; 149: 1419.
Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.
If you have published an article using product 21270, please notify us so that we can cite your literature.