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eIF2a(Ab-51) Antibody#21271

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Product Detail

Product NameeIF2a(Ab-51) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total eIF2a protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.49~53 (E-L-S-R-R) derived from Human eIF2a.

Target NameeIF2a

Other NamesEukaryotic translation initiation factor 2 subunit alpha; EIF-2A;

Accession NoSwiss-Prot: P05198
NCBI Protein: NP_004085.1

Uniprot P05198

Gene ID 1965;

Concentration0.6mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 38kd
Western blotting: 1:200~1:1000
Immunohistochemistry: 1:30~1:150
 

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: A549 cell,Human placenta tissue,A172 cell,NIH/3T3 cell,Jurkat cell,Hela cell lysates Primary antibody: 21271(eIF2a Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 21271(eIF2a(Ab-51) Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 21271(EIF2S1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hong-Li Wu, Yu-Hua Li, Yan-Hua Lin, et al. (2008) Salvianolic acid B protects human endothelial cells from oxidative stress damage: a possible protective role of glucose-regulated protein 78 induction Cardiovasc Res doi:10.1093/cvr/cvn262
This article references the use of the #21271.

If you have published an article using product 21271, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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