Product Detail
Product NamePKR(Ab-446) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total PKR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.444~448 (K-R-T-R-S) derived from Human PKR.
Target NamePKR
ConjugateUnconjugated
Other NamesADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR
Accession NoSwiss-Prot: P19525
NCBI Protein: NP_001129123.1
Uniprot
P19525
Gene ID
5610;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293, Hela and C6 cells using PKR(Ab-446) Antibody #21272.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR(Ab-446) Antibody #21272(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PKR(Ab-446) Antibody #21272.
Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.
Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734 - 18745.
If you have published an article using product 21272, please notify us so that we can cite your literature.
et al,Fluoxetine Inhibits NLRP3 Inflammasome Activation: Implication in Depression.In Int J Neuropsychopharmacol.On 2016 Sep 21 by Du RH, Tan J et al..PMID:27207922
, (2016),
PMID:
27207922
Yes
