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GAP43(Ab-41) Antibody#21273

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Product Detail

Product NameGAP43(Ab-41) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total GAP43 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.38~43 (Q-A-S-F-R) derived from Human GAP43.

Target NameGAP43

ConjugateUnconjugated

Other NamesB-50; BASP2; NEUM; PP46; axonal membrane protein GAP-43

Accession NoSwiss-Prot: P17677
NCBI Protein: NP_001123536.1

Uniprot P17677

Gene ID 2596;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from mouse brain tissue using GAP43(Ab-41) Antibody #21273 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GAP43(Ab-41) Antibody #21273(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using GAP43(Ab-41) Antibody #21273.
GAP43 encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Rachael L. Neve, et,al. (1998) J. Neurosci; 18: 7757.
Yiping Shen, et,al. (2002) J. Neurosci; 22: 239.
Chantal Gamby, et,al. (1996) J. Biol. Chem; 271: 26698.

If you have published an article using product 21273, please notify us so that we can cite your literature.

et al,Responses of PKCε to cardiac overloads on myocardial sympathetic innervation and NET expression.In Auton Neurosci. On 2018 Mar by Hu B, Zhang J et al..PMID:29195789 , (2018),
PMID: 29195789

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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