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Integrin b3(Ab-785) Antibody#21274

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Product Detail

Product NameIntegrin b3(Ab-785) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Integrin b3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.783~787 (I-T-Y-R-G) derived from Human Integrin B3/ITGB3.

Target NameIntegrin b3

ConjugateUnconjugated

Other NamesCD61 antigen; GP3A; GPIIIa; ITB3; Platelet membrane glycoprotein IIIa

Accession NoSwiss-Prot: P05106
NCBI Protein: NP_000203.2

Uniprot P05106

Gene ID 3690;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 110kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HL60 and HT29 cells using Integrin b3(Ab-785) Antibody #21274.

The Itg-Beta3 gene encodes the beta subunit of the platelet membrane adhesive protein receptor complex Itg-Beta3 protein, which belongs to the Integrin class of cell adhesion molecule receptors that share a common heterotrimeric structure with a and Beta subunits. It is a common Beta subunit of the platelet complex and of two other Integrins (Fibronectin and Vitronectin Receptors), which have distinct a subunits. Itg-Beta3 has been implicated in a wide variety of functions, including platelet aggregation and thrombosis and implantation, placentation, angiogenesis, bone remodeling, and tumor progression. Glanzmann Thrombasthenia can result from defects in the genes for either the Itg-a2B or the Itg-Beta3 subunit

Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397(Pt 3): 437

If you have published an article using product 21274, please notify us so that we can cite your literature.

et al,Lipid raft regulates the initial spreading of melanoma A375 cells bymodulating 1 integrin clustering. , (2013),
PMID: 23665237
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et al..PMID: 23665237 , (2013),
PMID: 23665237

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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