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a-Synuclein(Ab-133) Antibody#21277

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Product Detail

Product Namea-Synuclein(Ab-133) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Synuclein protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 131~135 (E-G-Y-Q-D) derived from Human a-Synuclein.

Target Namea-Synuclein

ConjugateUnconjugated

Other NamesNACP; SYN; SYUA; alpha-synuclein;

Accession NoSwiss-Prot: P37840
NCBI Protein: NP_000336.1

Uniprot P37840

Gene ID 6622;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using a-Synuclein(Ab-133) Antibody #21277.
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Ab-133) Antibody #21277.
May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Kozikowski AP, et al. (2006)ChemMedChem. 1(2):256-66.
Jakowec MW,et al.(2001)Dev Neurosci.23(2):91-9.
El-Agnaf OM,et al.(1998)FEBS Lett. 440(1-2):67-70.

If you have published an article using product 21277, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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