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a-Synuclein(Ab-136) Antibody#21278

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Product Detail

Product Namea-Synuclein(Ab-136) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total a-Synuclein protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 134~138 (Q-D-Y-E-P) derived from Human α-Synuclein.

Target Namea-Synuclein

ConjugateUnconjugated

Other NamesNACP; SYN; SYUA; alpha-synuclein;

Accession NoSwiss-Prot: P37840
NCBI Protein: NP_000336.1

Uniprot P37840

Gene ID 6622;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Hela and 3T3 cells using a-Synuclein(Ab-136) Antibody #21278.
Immunofluorescence staining of methanol-fixed Hela cells using a-Synuclein(Ab-136) Antibody #21278.

SncA is a member of the synuclein family of structurally related proteins that are prominently expressed in the central nervous system, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and SncA and Snc-Beta inhibit phospholipase D2 selectively. SncA may serve to integrate presynaptic signaling and membrane trafficking. Aggregated SncA proteins form brain lesions that are hallmarks of neurodegenerative synucleinopathies. Defects in SncA play a role in the pathogenesis of Parkinson disease. SncA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer disease. SncA shares 95% sequence homology with rat SncA. Rat SncA is specifically expressed in brain and is associated with synaptosomal membranes in neurons

Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119. 

If you have published an article using product 21278, please notify us so that we can cite your literature.

et al,Expression and Subcellular Location of Alpha-Synuclein During Mouse-Embryonic Development , (2010),
PMID: 19885730
et al,Expression and Subcellular Location of Alpha-Synuclein During Mouse-Embryonic Development. In Cell Mol Neurobiol on 2010 Apr by Shan-chuan Zhong, Xue Luo, et al..PMID: 19885730 , (2010),
PMID: 19885730
et al,Alpha-synuclein loss in spinal muscular atrophy. In J Mol Neurosci on 2011 Mar by Gyula Acsadi, Xingli Li, et al..PMID: 20640532 , (2011),
PMID: 20640532
et al,Distribution of the Alpha-Synuclein in the Brain and the Primary Organs of the Rhesus Monkey. In Appl Biochem Biotechnol on 2021 Oct by Jin-Ling Yang, Jia-Hong Gao, et al..PMID:34097253 , (2021),
PMID: 34097253

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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