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PKR(Ab-451) Antibody#21282

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Product Detail

Product NamePKR(Ab-451) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsIHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total PKR protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.449~453 (K-G-T-L-R) derived from Human PKR.

Target NamePKR

ConjugateUnconjugated

Other NamesADRB2; E2AK2; EIF2AK2; EIF2aK; PRKR

Accession NoSwiss-Prot: P19525
NCBI Protein: NP_001129123.1

Uniprot P19525

Gene ID 5610;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 68kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR(Ab-451) Antibody #21282(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PKR(Ab-451) Antibody #21282.

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Ingrid K. Ruf, et,al. (2005) J. Virol ; 79: 14562 - 14569.
Christy M. Hebner,et,al. (2006) J. Gen. Virol ; 87: 3183 - 3193.
Rika van Huizen, et,al. (2003) J. Biol. Chem ; 278: 15558 - 15564.

If you have published an article using product 21282, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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