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MARCKS(Ab-158) Antibody#21285

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Product Detail

Product NameMARCKS(Ab-158) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total MARCKS protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.156~160 (R-F-S-F-K) derived from Human MARCKS.

Target NameMARCKS

ConjugateUnconjugated

Other NamesMACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate

Accession NoSwiss-Prot: P29966
NCBI Protein: NP_002347.5

Uniprot P29966

Gene ID 4082;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells using MARCKS(Ab-158) Antibody #21285 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using MARCKS(Ab-158) Antibody #21285.
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159

If you have published an article using product 21285, please notify us so that we can cite your literature.

et al,Signaling pathways mediating phosphorylation and inactivation of glycogen synthase kinase-3b by the recombinant human d-opioid receptor stably expressed in Chinese hamster ovary cells. , (2011),
PMID: 21276805
et al,Signaling pathways mediating phosphorylation and inactivation of glycogen synthase kinase-3尾 by the recombinant human 未-opioid receptor stably expressed in Chinese hamster ovary cells. In Neuropharmacology on 2011 Jun by Maria C Olianas, Simona Dedoni, et al..PMID: 21276805 , (2011),
PMID: 21276805
et al,Phosphoproteome of signaling by ErbB2 in ovarian cancer cells. In Biochim Biophys Acta Proteins Proteom on 2022 Apr 1 by C Sidhanth, S Bindhya,et al..PMID:35158093 , (2022),
PMID: 35158093

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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