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NFkB-p100(Ab-872) Antibody#21297

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Product Detail

Product NameNFkB-p100(Ab-872) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total NFkB-p100 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFkB-p100.

Target NameNFkB-p100

ConjugateUnconjugated

Other NamesDNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2

Accession NoSwiss-Prot: Q00653
NCBI Protein: NP_001070961.1

Uniprot Q00653

Gene ID 4791;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52(Ab-872) Antibody #21297(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p100(Ab-872) Antibody #21297.
Western blot analysis of extracts from HT29 cells using NFkB-p100(Ab-872) Antibody #21297 and the same antibody preincubated with blocking peptide.

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Shin HM, et al. (2006) EMBO J; 25(1): 129-138.
Li Q, et al. (2005) Proc Natl Acad Sci USA; 102(35): 12425-12430.
Chen C, et al. (2000) Mol Cell Biol; 20(8): 2687-2695.
 

If you have published an article using product 21297, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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