Product Detail
Product NameCREB(Ab-142) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsIHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total CREB protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.140~144 (D-L-S-S-D) derived from Human CREB.
Target NameCREB
ConjugateUnconjugated
Other NamesCREB-1; CREB1;
Accession NoSwiss-Prot: P16220
NCBI Protein: NP_004370.1
Uniprot
P16220
Gene ID
1385;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 43kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-142) Antibody #21300(left) or the same antibody preincubated with blocking peptide(right).
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948.
Lilah Rothem,et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543.
Darren R. Tyson, et,al. (2002) Endocrinology; 143: 674.
Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.
If you have published an article using product 21300, please notify us so that we can cite your literature.
Yes
