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GSK3α/β(Ab-279/216) Antibody#21301

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Product Detail

Product NameGSK3α/β(Ab-279/216) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total GSK3α/β protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.277~281/214~218 (V-S-Y-I-C) derived from Human GSK3α/β.

Target NameGSK3α/β

ConjugateUnconjugated

Other NamesFactor A; GSK-3 alpha/beta; kinase GSK3-alpha/beta

Accession NoSwiss-Prot:P49840
Gene ID:2931

Uniprot P49840

Gene ID 2931;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 46, 51kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HL60, HT29 and 293 cells using GSK3α/β(Ab-279/216) Antibody #21301.

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137(1-2): 193-201
Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316(2): 411-415
Fan G, et al. J Biol Chem 2003 Dec 26; 278(52): 52432-52436
Liao X, et al. Mol Cancer Ther 2003 Nov; 2(11): 1215-1222

If you have published an article using product 21301, please notify us so that we can cite your literature.

et al,Cleavage of GSK-3尾 by Calpain Counteracts the Inhibitory Effect of Ser9 Phosphorylation on GSK-3尾 Activity Induced by H鈧侽锟?In J Neurochem on 2013 Jul by Ye Feng, Yiyuan Xia,et al..PMID:23646926 , (2013),
PMID: 23646926

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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