Product Detail
Product NameIGF-1R(Ab-1280) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total IGF-1R protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 1278~1282 (S-F-Y-Y-S ) derived from Human IGF-1R .
Target NameIGF-1R
ConjugateUnconjugated
Other Names Insulin-like growth factor I receptor
Accession NoSwiss-Prot: P08069
NCBI Protein: NP_000866.1
Uniprot
P08069
Gene ID
3480;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 200 95 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293,3T3 and Hela cells using IGF-1R(Ab-1280) Antibody #21302.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R(Ab-1280) Antibody #21302(left) or the same antibody preincubated with blocking peptide(right).
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.
Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093
Baserga, R. et al. (2000) Oncogene 19, 5574-5581
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
O'Connor R, et al. Mol Cell Biol 1997 Jan; 17(1): 427-35
If you have published an article using product 21302, please notify us so that we can cite your literature.
et al,Sex differences and left-right asymmetries in expression of insulin and insulin-like growth factor-1 receptors in developing rat
hippocampus.In Brain Struct Funct on 2012 Apr by Hami J, Sadr-Nabavi A,et al..PMID:22042446
, (2012),
PMID:
22042446
et al,Clinical significance of immunohistochemical expression of insulin-like growth factor-1 receptor and matrix metalloproteinase-7
in resected non-small cell lung cancer.In Exp Ther Med on 2012 May by Yamamoto T, Oshima T,et al..PMID:22969971
, (2012),
PMID:
22969971
Yes
