Product Detail
Product NameIGF-1R(Ab-1346) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total IGF-1R protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.1344~1348 (Q-P-Y-A-H) derived from Human IGF-1R .
Target NameIGF-1R
ConjugateUnconjugated
Other Names Insulin-like growth factor I receptor; CD221; IGF1R; kinase IGF1R;
Accession NoSwiss-Prot: P08069
NCBI Protein: NP_000866.1
Uniprot
P08069
Gene ID
3480;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 200 95 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using IGF-1R(Ab-1346) Antibody #21303 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IGF-1R(Ab-1346) Antibody #21303(left) or the same antibody preincubated with blocking peptide(right).
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.
Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093
Baserga, R. et al. (2000) Oncogene 19, 5574-5581
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
O'Connor R, et al. Mol Cell Biol 1997 Jan; 17(1): 427-35
If you have published an article using product 21303, please notify us so that we can cite your literature.
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