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IKK-b(Ab-199) Antibody#21305

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Product Detail

Product NameIKK-b(Ab-199) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total IKK-bprotein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b.

Target NameIKK-b

ConjugateUnconjugated

Other NamesI-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IkBKB; NFKBIKB

Accession NoSwiss-Prot: O14920
NCBI Protein: NP_001547.1

Uniprot O14920

Gene ID 3551;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 87kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MDA cells using IKK-b(Ab-199) Antibody #21305.
Immunofluorescence staining of methanol-fixed Hela cells using IKK-b(Ab-199) Antibody #21305.
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.

Chen RA, et al. PLoS Pathog. 2008 Feb 8;4(2):e22
Yen CJ,et al. Cancer Res. 2008 Apr 15;68(8):2632-40

If you have published an article using product 21305, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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