Product Detail
Product NameKeratin 8(Ab-74) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Keratin 8 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8).
Target NameKeratin 8
ConjugateUnconjugated
Other NamesCK 8; CK8; CYK8; Cytokeratin endo A; K8
Accession NoSwiss-Prot: P05787
NCBI Protein: NP_002264.1
Uniprot
P05787
Gene ID
3856;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 55kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HUVEC and 293 cells using Keratin 8(Ab-74) Antibody #21307.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8(Ab-74) Antibody #21307(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8(Ab-74) Antibody #21307.
Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751.
If you have published an article using product 21307, please notify us so that we can cite your literature.
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