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MAPKAPK-2(Ab-334) Antibody#21308

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Product Detail

Product NameMAPKAPK-2(Ab-334) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total MAPKAPK-2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.332~336 (P-Q-T-P-L) derived from Human MAPKAPK-2.

Target NameMAPKAPK-2

ConjugateUnconjugated

Other NamesMAP kinase-activated protein kinase 2

Accession NoSwiss-Prot: P49137
NCBI Protein: NP_004750.1

Uniprot P49137

Gene ID 9261;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 49kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 cells using MAPKAPK-2(Ab-334) Antibody #21308 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Ab-334) Antibody #21308.
Western blot analysis of extracts from JK, 3T3 and C6 cells using MAPKAPK-2 (Ab-334) Antibody #21308

MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Rouse, J. et al. (1994) Cell 78, 1027-1037.
Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

If you have published an article using product 21308, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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