Product Detail
Product NamePKCz(Ab-410) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total PKCz protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCz
Target NamePKCz
ConjugateUnconjugated
Other NamesPKC2; PKC-ZETA; PRKCZ
Accession NoSwiss-Prot: Q05513
NCBI Protein: NP_002735.3
Uniprot
Q05513
Gene ID
5590;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 78kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extract from 293 cells and Hela using PKCz(Ab-410) Antibody #21314.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCz(Ab-410) antibody(#21314).
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4.
Castrillo A, et al. (2003) Mol Cell Biol; 23(4): 1196-1208.
Paramio JM, et al. ( 2001) Mol Cell Biol; 21(21): 7449-7459.
Braiman L, et al. (2001) Mol Cell Biol; 21(22): 7852-7861.
If you have published an article using product 21314, please notify us so that we can cite your literature.
Yes
