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SHP-2(Ab-580) Antibody#21320

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Product Detail

Product NameSHP-2(Ab-580) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total SHP-2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.578~582 (R-V-Y-E-N) derived from Human SHP-2.

Target NameSHP-2

ConjugateUnconjugated

Other NamesProtein-tyrosine phosphatase 2C

Accession NoSwiss-Prot: Q06124
NCBI Protein: NP_002825.3

Uniprot Q06124

Gene ID 5781;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using SHP-2(Ab-580) Antibody #21320.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-2(Ab-580) Antibody #21320(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using SHP-2(Ab-580) Antibody #21320.
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928.
Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536.

If you have published an article using product 21320, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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