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Smad2(Ab-467) Antibody#21322

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Product Detail

Product NameSmad2(Ab-467) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Smad2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 463~467 (C-S-S-M-S) derived from Human Smad2.

Target NameSmad2

ConjugateUnconjugated

Other NamesMad-related protein 2

Accession NoSwiss-Prot: Q15796
NCBI Protein: NP_001003652.1

Uniprot Q15796

Gene ID 4087;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using Smad2(Ab-467) Antibody #21322.
Immunofluorescence staining of methanol-fixed Hela cells using Smad2(Ab-467) Antibody #21322.
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

If you have published an article using product 21322, please notify us so that we can cite your literature.

et al,High glucose increases Cdk5 activity in podocytes via transforming growth factor-尾1 signaling pathway.In Exp Cell Res on 2014 Aug 15 by Yue Zhang, Hongbo Li et al..PMID: 24768698 , (2014),
PMID: 24768698

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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