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Smad2(Ab-220) Antibody#21323

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Product Detail

Product NameSmad2(Ab-220) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Smad2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.

Target NameSmad2

ConjugateUnconjugated

Other NamesJV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2

Accession NoSwiss-Prot: Q15796
NCBI Protein: NP _001003652.1

Uniprot Q15796

Gene ID 4087;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 and 3T3 cells using Smad2(Ab-220) Antibody #21323
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad2(Ab-220) Antibody #21323(left) or the same antibody preincubated with blocking peptide(right).
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Sang Gyun Kim et al. (2004) Mol Biol Cell. February; 15(2): 420

If you have published an article using product 21323, please notify us so that we can cite your literature.

et al,Distribution of Smad mRNA and proteins in the rat brain.In J Chem Neuroanat. On 2018 Jul by Nakajima T, Hata R et al..PMID: 29196107 , (2018),
PMID: 29196107
et al,TGF β1 mediated Smad signaling pathway and EMT in hepatic fibrosis induced by Nano NiO in vivo and in vitro.In Environ Toxicol on 2020 Apr by Zhang Q, Chang X ,et al..PMID: 31737983 , (2020),
PMID: 31737983
et al,Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.In J Neurol Sci on 2014 Feb 15 by Takayuki Nakajima , Masafumi Yanagihara et al ..PMID:24290497 , (2014),
PMID: 24290497

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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