Product NameSmad3(Ab-425) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Smad3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.423~427 (C-S-S-V-S) derived from Human Smad3.

Target NameSmad3

ConjugateUnconjugated

Other NamesJV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3

Accession NoSwiss-Prot: P84022
NCBI Protein: NP_001138574.1

Uniprot P84022

Gene ID 4088;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 52kd

Western blotting1:500~1:1000

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200