Product Detail
Product Namea-catenin(Ab-641) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total a-catenin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 639~643 (D-D-S-D-F) derived from Human a-catenin.
Target Namea-catenin
ConjugateUnconjugated
Other NamesCadherin-associated protein; Alpha E-catenin; NY-REN-13 antigen
Accession NoSwiss-Prot: P35221
NCBI Protein: NP_001894.2
Uniprot
P35221
Gene ID
1495;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 100kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela and 293 cells using a-catenin(Ab-641) antibody #21330.
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.
Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765
Drees, F. et al. (2005) Cell 123, 903-915.
Yamada, S. et al. (2005) Cell 123, 889-901.
Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.
If you have published an article using product 21330, please notify us so that we can cite your literature.
et al,伪-Catenin phosphorylation promotes intercellular adhesion through a dual-kinase mechanism. In J Cell Sci on 2015 Mar 15 by David J Escobar, Ridhdhi Desai,et al..PMID:25653389
, (2015),
PMID:
25653389
et al,The Actin-Binding Protein Alpha-Catenin is Phosphorylated by a Dual-Kinase Mechanism to Promote Intercellular Adhesion and Density-Dependent Inhibition of Proliferation
, (2015),
PMID:
25653389
Yes
