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LC3a Antibody#21402

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Product Detail

Product NameLC3a Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total LC3A protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.5~10 (R-P-F-K-Q) derived from Human LC3A.

Target NameLC3a

ConjugateUnconjugated

Other NamesMicrotubule-associated protein 1 light chain 3 alpha; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1 light chain 3-like protein 1; Autophagy-related protein LC3 A

Accession NoSwiss-Prot: Q9H492
NCBI Protein: NP_115903.1

Uniprot Q9H492

Gene ID 84557;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 18 16 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela and 293 cells using LC3A antibody #21402.
Immunofluorescence staining of methanol-fixed Hela cells using LC3A antibody #21402.
Probably involved in formation of autophagosomal vacuoles (autophagosomes).

If you have published an article using product 21402, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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