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Melanoma-associated antigen D1 Antibody#21408

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Product Detail

Product NameMelanoma-associated antigen D1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total Melanoma-associated antigen D1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide with sequence C-EAEARAEARNRMGIGDE derived from Mouse Melanoma-associated antigen D1.

Target NameMelanoma-associated antigen D1

ConjugateUnconjugated

Other NamesMAGED1

Accession NoSwiss-Prot: Q9QYH6
NCBI Protein: NP_062765.1

Uniprot Q9QYH6

Gene ID 94275;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 95kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from PC12 cells using Melanoma-associated antigen D1 Antibody #21408 and the same antibody preincubated with blocking peptide.

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Binds p75NTR and antagonizes its association with TrkA, inhibits cell cycle progression, and facilitates p75NTR-mediated apoptosis. May act as a regulator of the function of DLX family members. May regulate p53 transcriptional activity and inhibit cell proliferation. Enhances p53 phosphorylation and accumulation.

Zhao Z,et al.(2002) Sci China C Life Sci.45(4):412-20.
Shen WG,et al.(2007) Cell Commun Adhes.14(1):21-31.
Cheresh DA,et al. (1984) J Biol Chem.259(12):7453-9.

If you have published an article using product 21408, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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