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BMAL1 Antibody#21415

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Product Detail

Product NameBMAL1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total BMAL1 protein.

Immunogen TypePeptide-KLH

Immunogen DescA synthesized peptide derived from human BMAL1

Target NameBMAL1

ConjugateUnconjugated

Other NamesMOP3; BMAL1c; ARNTL; TIC;

Accession NoSwiss-Prot: O00327
NCBI Protein: NP_001025443.1

Uniprot O00327

Gene ID 406;

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Application Details

Predicted MW: 69kd
WB 1:1000-1:2000

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of BMAL1 expression in human fetal heart lysate.
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes.

If you have published an article using product 21415, please notify us so that we can cite your literature.

et al,Dual roles of FBXL3 in the mammalian circadian feedback loops are important for period determination and robustness of the clock. In Proc Natl Acad Sci U S A on 2013 Mar 19 by Guangsen Shi, Lijuan Xing, et al..PMID: 23471982 , (2013),
PMID: 23471982
et al,Diurnal Control of Blood Pressure Is Uncoupled From Sodium Excretion. In Hypertension on 2020 Jun by Jermaine G Johnston, Joshua S Speed, et al..PMID:32306766 , (2020),
PMID: 32306766
et al,Dysregulated clock gene expression and abnormal diurnal regulation of hippocampal inhibitory transmission and spatial memory in amyloid precursor protein transgenic mice. In Neurobiol Dis on 2021 Oct by Allison R Fusilier, Jennifer A Davis,et al..PMID:34333153 , (2021),
PMID: 34333153

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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