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H2B Antibody#21417

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Product Detail

Product NameH2B Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total H2B protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.

Target NameH2B

ConjugateUnconjugated

Other NamesH2BQ; GL105; H2B.1; H2BFQ; H2BGL105

Accession NoSwiss-Prot: P33778
NCBI Protein: NP_003519.1

Uniprot P33778

Gene ID 3018;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 14kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela and 293 cells using H2B antibody #21417.

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Borchert GM,et al. (2010) PLoS One.5(7):e11641.
Pennini ME,et al.(2010) PLoS Pathog.6(7):e1000995.
Lu C,et al. (2010) Sci China Life Sci.53(6):663-8.

If you have published an article using product 21417, please notify us so that we can cite your literature.

et al,The Eaf3/5/7 subcomplex stimulates NuA4 interaction with methylated histone H3 Lys-36 and RNA polymerase II.In J Biol Chem on 2016 Sep 30 by Jake M Lippi, Leah Cohen,et al..PMID:27535225 , (2016),
PMID: 27535225

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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