Product Detail
Product NameFas Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Fas protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.323~327 (E-N-S-N-F) derived from human Fas .
Target NameFas
ConjugateUnconjugated
Other NamesFASLG receptor; Apoptosis-mediating surface antigen FAS; Apo-1 antigen
Accession NoSwiss-Prot:
P25445
NCBI Protein: NP_000034.1
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 40-50kd
Western blotting: 1:1000
Western blot analysis of extracts from HepG2, Hela and 293 cells using Fas Antibody #21419.
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
Nagata, S. (1997) Cell 88, 355-65.
Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.
If you have published an article using product 21419, please notify us so that we can cite your literature.
et al,Activation of Fas death receptor pathway and Bid in hepatocytes is involved in saikosaponin D induction of hepatotoxicity.In Environ Toxicol Pharmacol on 2016 Jan by Feng Zhang , Li Chen et al..PMID:26645133
, (2016),
PMID:
26645133
Yes
