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Caspase 3 Antibody#21420

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Product Detail

Product NameCaspase 3 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total Caspase 3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.

Target NameCaspase 3

ConjugateUnconjugated

Other NamesApopain

Accession NoSwiss-Prot: P42574
NCBI Protein: NP_004337.2

Uniprot P42574

Gene ID 836;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 35 17kd
Western blotting: 1:1000

Western blot analysis of extracts from 293, Hela and MDA cells using Caspase 3 Antibody #21420.

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Gervais, F. G et al.Cell 97: 395-406, 1999.
Nicholson, D. W. et al. (1995) Nature 376, 37-43.

If you have published an article using product 21420, please notify us so that we can cite your literature.

et al,BAD overexpression inhibits cell growth and induces apoptosis via mitochondrial-dependent pathway in non-small cell lung cancer.In Cancer Cell Int on 2013 Jun 1 by Jiang L, Luo M, et al..PMID:23725574 , (2013),
PMID: 23725574
et al,Ampelopsin induces apoptosis in HepG2 human hepatoma cell line through extrinsic and intrinsic pathways: Involvement of P38 and ERK.In Environ Toxicol Pharmacol on 2015 Nov by Shimei Qi, Xianjuan Kou et al..PMID: 26476886 , (2015),
PMID: 26476886
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987 , (2012),
PMID: 23191987

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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