Product Detail
Product NameCaspase8 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Caspase 8 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.217~221 (S-E-S-Q-T) derived from Caspase 8.
Target NameCaspase-8
ConjugateUnconjugated
Other NamesICE-like apoptotic protease 5
Accession NoSwiss-Prot: Q14790
NCBI Protein: NP_001219.2
Uniprot
Q14790
Gene ID
841;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 18,43,57kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from 293, Hela and HT29 cells using Caspase8 Antibody #21421
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Himeji D.et.al.(2002)Blood 99:4070-4078
Breckenridge D.G.et.al. (2002)Proc. Natl. Acad. Sci. U.S.A. 99:4331-4336
Muzio M.et.al. (1997)J. Biol. Chem. 272:2952-2956
If you have published an article using product 21421, please notify us so that we can cite your literature.
et al,BAD overexpression inhibits cell growth and induces apoptosis via mitochondrial-dependent pathway in non-small cell lung cancer.In Cancer Cell Int on 2013 Jun 1 by Jiang L, Luo M, et al..PMID:23725574
, (2013),
PMID:
23725574
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987
, (2012),
PMID:
23191987
Yes
