Product Detail
Product NameCaspase 9 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Caspase 9 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9
Target NameCaspase 9
ConjugateUnconjugated
Other NamesICE-like apoptotic protease 6
Accession NoSwiss-Prot: P55211
NCBI Protein: NP_001220.2
Uniprot
P55211
Gene ID
842;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 47 37 35kd
Western blotting: 1:1000
Western blot analysis of extracts from 293 and Hela cells using Caspase 9 Antibody #21422.
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
Li, P. et al. (1997) Cell 91, 479-489.
If you have published an article using product 21422, please notify us so that we can cite your literature.
et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID:
23440283
, (2013),
PMID:
23440283
Yes
