Product Detail
Product NameNanog Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total Nanog protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g
Target NameNanog
ConjugateUnconjugated
Other NamesHomeobox transcription factor Nanog
Accession NoSwiss-Prot: Q9H9S0
NCBI Protein: NP_079141.2
Uniprot
Q9H9S0
Gene ID
79923;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 42kd
Western blotting: 1:1000
Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using Nano g Antibody #21423.
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation
Do HJ, et al.Biochem Biophys Res Commun. 2007 Feb 16;353(3):770-5.
Boyer LA, et al.Cell. 2005 Sep 23;122(6):947-56.
Freberg CT, et al. Mol Biol Cell. 2007 May;18(5):1543-53.
Chambers I, et al. Cell. 2003 May 30;113(5):643-55.
If you have published an article using product 21423, please notify us so that we can cite your literature.
et al,Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness.In BMC Cancer on 2015 Oct 24 by Jianguo Xue , Yuan Zhu et al..PMID: 26498753
, (2015),
PMID:
26498753
et al,SALL4 promotes gastric cancer progression through activating CD44 expression.In Oncogenesis on 2016 Nov 7 by X Yuan , X Zhang et al..PMID:27819668
, (2016),
PMID:
27819668
et al,HucMSC exosome-delivered 14-3-3ζ orchestrates self-control of the Wnt response via modulation of YAP during cutaneous regeneration. In Stem Cells on 2016 Oct by Bin Zhang, Yinghong Shi, et al..PMID: 27334574
, (2016),
PMID:
27334574
Yes
