Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > OCT-4 Antibody

OCT-4 Antibody#21424

Availability: Yes
Total Amount:$
Product Detail

Product NameOCT-4 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total OCT-4 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4

Target NameOCT-4

ConjugateUnconjugated

Other NamesOtc3; OTF3; OTF4; OTF-3;

Accession NoSwiss-Prot: Q01860
NCBI Protein: NP_002692.2

Uniprot Q01860

Gene ID 5460;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 45kd
Western blotting: 1:1000
 

Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using OCT-4 Antibody #21424.

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278.
Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
 

If you have published an article using product 21424, please notify us so that we can cite your literature.

et al,Mouse bone marrow mesenchymal stem cells with distinct p53 statuses display differential characteristics.In Mol Med Rep on 2020 May;21 by Wang B, Wang L, et al..PMID: 32186775 , (2020),
PMID: 32186775
et al,Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway.In Mol Med Rep.On 2016 Oct by Gu H, Ji R et al..PMID:27513187 , (2016),
PMID: 27513187

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;