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SOX2 Antibody#21425

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Product Detail

Product NameSOX2 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total SOX2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.76~80 (G-A-E-W-K) derived from SOX2

Target NameSOX2

ConjugateUnconjugated

Other NamesTranscription factor SOX-2

Accession NoSwiss-Prot: P48431
NCBI Protein: NP_003097.1

Uniprot P48431

Gene ID 6657;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 35kd
Western blotting: 1:1000
Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using SOX2 Antibody #21425.
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

Yuan H, et al. Genes Dev. 1995 Nov 1;9(21):2635-45
Muta M, et al. Genes Cells 2002 Aug;7(8):791-805
Tomioka M, et al. Nucleic Acids Res. 2002 Jul 15; 30(14): 3202-3213.

If you have published an article using product 21425, please notify us so that we can cite your literature.

et al,Activation of mesenchymal stem cells by macrophages prompts human gastric cancer growth through NF-魏B pathway.In PLoS One on 2014 May 13 by Tingting Yang , Xu Zhang et al..PMID:24824968 , (2014),
PMID: 24824968
et al,SALL4, a novel marker for human gastric carcinogenesis and metastasis.In Oncogene on 2014 Nov 27 by L Zhang , Z Xu et al..PMID:24276240 , (2014),
PMID: 24276240
et al,Tumorigenic hybrids between mesenchymal stem cells and gastric cancer cells enhanced cancer proliferation, migration and stemness.In BMC Cancer on 2015 Oct 24 by Jianguo Xue , Yuan Zhu et al..PMID: 26498753 , (2015),
PMID: 26498753

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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