Product Detail
Product NameEWS Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous levels of total EWS protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.213~217(T-Y-G-Q-P)derived from Human EWS.
Target NameEWS
ConjugateUnconjugated
Other NamesbK984G1.4; EWSR1;
Accession NoSwiss-Prot: Q9BZD1
NCBI Protein: NP_053733.2
Uniprot
Q9BZD1
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 85kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 293 and 3T3 cells using EWS Antibody #21432.
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Ubhi T.B.S., Rainey D.R., Craig A.R., Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
If you have published an article using product 21432, please notify us so that we can cite your literature.
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