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CDK4 Antibody#21437

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Product Detail

Product NameCDK4 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous levels of total CDK4 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4.

Target NameCDK4

ConjugateUnconjugated

Other NamesPSK-J3; CMM3; CMM3

Accession NoSwiss-Prot: P11802
NCBI Protein: NP_000066.1

Uniprot P11802

Gene ID 1019;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry:1:50~1:100
Western blot analysis of extracts from HUVEC cells using CDK4 Antibody #21437.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue usingCDK4 Antibody #21437.

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .

Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
Sherr, C.J. (1996) Science 274, 1672-1677.
Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.

If you have published an article using product 21437, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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