Product Detail
Product NameGRASP Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total GRASP protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 814-818(Q-E-I-V-R ) derived from Rat GRASP.
Target NameGRASP
ConjugateUnconjugated
Other NamesGripap; Grasp; GRIP1-associated protein
Accession NoSwiss-Prot: Q9JHZ4
NCBI Protein: NP_064522.3
Uniprot
Q9JHZ4
Gene ID
116493;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 95kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from rat brain and mouse brain tissue using GRASP Antibody #21449
GRASP (GRP1-associated scaffold protein, tamalin) is a 395 amino acid protein
encoded by the human gene GRASP . GRASP is a scaffold protein that com-prises multiple protein-interacting domains, including a 95 kDa postsynaptic density protein (PSD-95)/discs-large/ZO-1 (PDZ) domain, a leucine-zipper
region and a carboxyl-terminal PDZ-binding motif. GRASP is involved with intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses. GRASP forms a heteromer composed of GRASP , PSCD2 and at least one mGluR-1. It also interacts with PSCD3, mGluR-2, mGluR-3 and mGluR-5. GRASP is highly expressed in brain and has lower levels of expression in lung, heart, embryo, kidney and ovary.
Kitano, J., Kimura, K., Yamazaki, Y., Soda, T., Shigemoto, R., Nakajima, Y.and Nakanishi, S. 2002. Tamalin, J. Neurosci. 22: 280-1289.
Hall, B.S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S.K. and Field, M.C. 2006. J. Biol. Chem. 281: 27600-27612.
Sugi, T., Oyama, T., Muto, T., Nakanishi, S., Morikawa, K. and Jingami, H. 2007. Crystal structures of autoinhibitory PDZ domain of Tamalin EMBO J.26: 2192-2205.
If you have published an article using product 21449, please notify us so that we can cite your literature.
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