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Eif1 Antibody#21466

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Product Detail

Product NameEif1 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityDm

SpecificityThe antibody detects endogenous levels of total Eif1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 4~8 (Q-N-L-N-T) derived from Drosophila Eif1

Target NameEif1

ConjugateUnconjugated

Other NamesA121; ISO1; SUI1; EIF1A;

Accession NoSwiss-Prot: P41567
NCBI Protein: NP_005792.1

Uniprot P41567

Gene ID 10209;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 13kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from whole fruit fly(drosophila melanogaster) tissue lysate using Eif1 Antibody #21466.
Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA

Sheikh M.S., Fernandez-Salas E., Yu M., Hussain A., Fornace A.J. Jr. J. Biol. Chem. 274:16487-16493(1999)
Mendell J.T., Medghalchi S.M., Lake R.G., Noensie E.N., Dietz H.C. Mol. Cell. Biol. 20:8944-8957(2000)

If you have published an article using product 21466, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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