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CD44 Antibody#21471

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Product Detail

Product NameCD44 Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total CD44 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.734~738(Q-N-V-D-M) derived from Human CD44.

Target NameCD44

ConjugateUnconjugated

Other NamesLHR; MDU2; MDU3; MIC4;

Accession NoSwiss-Prot: P16070
NCBI Protein: NP_000601.3

Uniprot P16070

Gene ID 960;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunofluorescence:1:100~1:200
Western blot analysis of extract from Hela cells using CD44 Antibody #21471
Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using CD44 Antibody #21471.

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Teriete P.,et.al(2004) Mol. Cell 13:483-496 Bartolazzi A. (2003)Melanoma Res. 13:325-337
Liu T.et.al (2005)J. Proteome Res. 4:2070-2080

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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