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E-Cadherin(CD324) Antibody#21473

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Product Detail

Product NameE-Cadherin(CD324) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total E-Cadherin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.864~868(E-W-G-N-R) derived from Human E-Cadherin.

Target NameE-Cadherin(CD324)

ConjugateUnconjugated

Other NamesCDH1; CDHE; UVO

Accession NoSwiss-Prot: P12830
NCBI Protein: NP_004351.1

Uniprot P12830

Gene ID 999;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 135kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from MCF-7 and HT29 cells using E-Cadherin(CD324) Antibody #21473
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin a-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Baki L.et.al(2001)Proc. Natl. Acad. Sci. U.S.A. 98:2381-2386
Makagiansar I.T.et.al(2002)J. Biol. Chem. 277:16002-16010
Agiostratidou G. (2006)J. Neurochem. 96:1182-1188

If you have published an article using product 21473, please notify us so that we can cite your literature.

et al,N鈥憁ethyl鈥慛鈥憂itro鈥慛'鈥憂itrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2鈥憁ediated migration.In Mol Med Rep On 2016 Feb by Jie Cai , Mei Wang et al..PMID:26648448 , (2016),
PMID: 26648448
et al, Octamer-binding protein 4 affects the cell biology and phenotypic transition of lung cancer cells involving 尾-catenin/E-cadherin complex degradation.In Mol Med Rep on 2015 Mar by Zhong-Shu Chen, Dong-Jin Ling et al..PMID: 25420671 , (2015),
PMID: 25420671
et al,Migration Suppression of Small Cell Lung Cancer by Polysaccharides from Nostoc commune Vaucher. In J Agric Food Chem on 2016 Aug 17 by Min Guo, Guo-Bin Ding,et al..PMID: 27465400 , (2016),
PMID: 27465400

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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