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N-Cadherin(CD325) Antibody#21474

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Product Detail

Product NameN-Cadherin(CD325) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total N-Cadherin protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.800~804(A-I-K-P-V) derived from Human N-Cadherin.

Target NameN-Cadherin(CD325)

ConjugateUnconjugated

Other NamesCDH2; CDCN; NCAD

Accession NoSwiss-Prot: P19022
NCBI Protein: NP_001783.2

Uniprot P19022

Gene ID 1000;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 140kd
Western blotting: 1:500
Western blot analysis of extract from HT29 cells using N-Cadherin(CD325) Antibody #21474
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density

Bhatt A.S. et.al(2005)Oncogene 24:5333-5343
Salomon D.et.al(1992)J. Cell Sci. 102:7-17
The MGC Project Team(2004)Genome Res. 14:2121-2127

If you have published an article using product 21474, please notify us so that we can cite your literature.

et al,H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells.In Int J Mol Med on 2013 Dec by Zhang Q, Wang M,et al..PMID:24145921 , (2013),
PMID: 24145921
et al, Octamer-binding protein 4 affects the cell biology and phenotypic transition of lung cancer cells involving 尾-catenin/E-cadherin complex degradation.In Mol Med Rep on 2015 Mar by Zhong-Shu Chen, Dong-Jin Ling et al..PMID: 25420671 , (2015),
PMID: 25420671
et al,Paeonol Suppresses Proliferation and Motility of Non-Small-Cell Lung Cancer Cells by Disrupting STAT3/NF-kB Signaling. In Front Pharmacol on 2020 Nov 12 by Lei Zhang, Wen-Xu Chen , et al..PMID: 33442382 , (2020),
PMID: 33442382
et al,Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. In Front Cell Dev Biol on 2020 Nov 25 by Guangying Cui, Huifen Wang, et al..PMID:33324633 , (2020),
PMID: 33324633

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;