Product Detail
Product NameREST Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total REST protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.873~877(R-E-E-A-S) derived from Human REST.
Target NameREST
ConjugateUnconjugated
Other NamesNRSF; XBR; REST
Accession NoSwiss-Prot: Q13127
NCBI Protein: NP_001180437.1
Uniprot
Q13127
Gene ID
5978;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 121kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from Hela cells using REST Antibody #21475
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Chong J.A.et.al. (1995)Cell 80:949-957
Schoenherr C.J.et.al.(1995)Science 267:1360-1363
Scholl T.et.al.(1996)J. Immunol. 156:1448-1457
Lunyak V.V.et.al. (2002)Science 298:1747-1752
If you have published an article using product 21475, please notify us so that we can cite your literature.
Yes
