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AML1(RUNX1) Antibody#21477

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Product Detail

Product NameAML1(RUNX1) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total AML1(RUNX1) protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.6~10(D-A-S-T-S) derived from Human AML1(RUNX1).

Target NameAML1(RUNX1)

ConjugateUnconjugated

Other NamesCBFA2; EVI-1; AMLCR1

Accession NoSwiss-Prot: Q01196
NCBI Protein: NP_001116079.1

Uniprot Q01196

Gene ID 861;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 55kd
Western blotting: 1:500~1:1000

Western blot analysis of extract from P19, Hela and 293 cells using AML1(RUNX1) Antibody #21477
Western blotting analysis using AML1(RUNX1) Antibody #21477.

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The a subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.Mol. Cell. Biol. 19:3635-3644(1999)
Pelletier N., Champagne N., Stifani S., Yang X.-J. Oncogene 21:2729-2740(2002)
Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.J. Biol. Chem. 279:19512-19522(2004)

If you have published an article using product 21477, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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