Product Detail
Product NameGFAP Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IF
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total GFAP protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 423~427 (E-S-K-Q-E) derived from Human GFAP.
Target NameGFAP
ConjugateUnconjugated
Other NamesFLJ45472
Accession NoSwiss-Prot: P14136
NCBI Protein: NP_002046.1
Uniprot
P14136
Gene ID
2670;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 50kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extract from Rat brain, Rat hippocampus tissue and C6 cells using GFAP Antibody #21485
Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using GFAP Antibody #21485.
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Bongcam-Rudloff E., Nister M., Betsholtz C., Wang J.-L., Stenman G., Huebner K., Croce C.M., Westermark B.Cancer Res. 51:1553-1560(1991) Nielsen A.L., Holm I.E., Johansen M., Bonven B., Jorgensen P., Jorgensen A.L.J. Biol. Chem. 277:29983-29991(2002)
If you have published an article using product 21485, please notify us so that we can cite your literature.
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