Product Detail
Product NameNSE Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total NSE protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.6~10(I-W-A-R-E)derived from Human NSE.
Target NameNSE
ConjugateUnconjugated
Other NamesENO2
Accession NoSwiss-Prot: P09104
NCBI Protein: NP_001966.1
Uniprot
P09104
Gene ID
2026;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 47kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from JK, HUVEC, HepG2 and Hela cells using NSE Antibody #21487
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
McAleese S.M., Dunbar B., Fothergill J., Hinks L., Day I.N.M.Eur. J. Biochem. 178:413-417(1988)
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Yes
