Product Detail
Product NameFibrillarin Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Fibrillarin protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.297~301( L-T-L-E-P) derived from Human Fibrillarin
Target NameFibrillarin
ConjugateUnconjugated
Other NamesFBL; FIB; FLRN; RNU3IP1;
Accession NoSwiss-Prot: P22087
NCBI Protein: NP_001427.2
Uniprot
P22087
Gene ID
2091;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 37kd
Western blotting: 1:500~1:1000
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody #21495
Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.
Lischwe M.A., Ochs R.L., Reddy R., Cook R.G., Yeoman L.C., Tan E.M., Reichlin M., Busch H. J. Biol. Chem. 260:14304-14310(1985)
Yanagida M., Hayano T., Yamauchi Y., Shinkawa T., Natsume T., Isobe T., Takahashi N.J. Biol. Chem. 279:1607-1614(2004)
Wang Y., Liu J., Zhao H., Lue W., Zhao J., Yang L., Li N., Du X., Ke Y.Biochim. Biophys. Acta 1773:863-868(2007)
If you have published an article using product 21495, please notify us so that we can cite your literature.
et al,Synthetic Tryptanthrin Derivatives Induce Cell Cycle Arrest and Apoptosis via Akt and MAPKs in Human Hepatocellular Carcinoma Cells. In Biomedicines on 2021 Oct 24 by Jing-Yan Gao, Chih-Shiang Chang, et al..PMID: 34829756
, (2021),
PMID:
34829756
Yes
